Cintia Gomez Limia

Postdoctoral Fellow at Nationwide Children's Hospital

Responsible for mammalian cell line development using CRISPR and transposon systems. Optimized protocols for transgenic cells (CHO, HEK293). Implemented high-throughput strategies to select genetically modified cells. Established iPSC lines and directed differentiation toward hematopoietic and neural lineages.

Postdoctoral Fellow at The Ohio State University

Designed engineered extracellular vesicle-based therapies for neural and hematopoietic applications; managed lab operations, trained students, and coordinated project tasks. Developed and characterized iPSC-derived neural and hematopoietic cell models; guided genetic modification strategies and translational planning.

Postdoctoral Fellow at Gustave Roussy Institute of Oncology

Performed gene mutation screening and validation of feeder-free iPSC-derived lineages; contributed to leukemia disease models; developed high-throughput screening workflows and co-authored several publications.

Postdoctoral Fellow at IDOR – Institute for Research and Education / INCA

Generated and characterized patient-derived iPSCs to model disease states; implemented genome-editing strategies to study disease-related mutations; expanded iPSC cultures and evaluated in vitro differentiation strategies.

Scientist in Mammalian Cell Line Development at Demeetra

Responsible for mammalian cell line development using CRISPR and transposon systems. Executed knock-in/knockout genome editing strategies in CHO cells expressing antibodies. Performed single-cell cloning of CHO cells and implemented high-throughput systems to select genetically modified cells. Engineered T cells using transposase/transposon system to generate CAR-T cells, followed by single-cell cloning. Constructed plasmids and developed associated molecular strategies. Trained team members for cell culture and T7 assay. Wrote protocols, technical reports, and SOPs to ensure robust lab operations and reproducibility.

Postdoctoral Fellow at Nationwide Children’s Hospital

Focused on generating patient-derived induced pluripotent stem cells (iPSCs) and modeling Prune Belly Syndrome (PBS) in vitro to investigate the biological impact of specific human gene mutations and their correlation with disease severity. Established a new iPSC research line, generated and characterized human iPSCs using optimized reprogramming strategies to enhance efficiency.

Postdoctoral Fellow at Ohio State University

Designed a novel engineered extracellular vesicle therapy for neurofibromatosis. Developed engineered extracellular vesicle formulations by cell programming strategies to improve peripheral nervous system delivery in vivo and in vitro. Monitored mouse colony expansion, genotype and phenotype analyses, and supervised student researchers; documented protocols and supported lab organization and workflow efficiency.

Postdoctoral Fellow at Gustave Roussy Institute of Oncology

Performed gene mutation screening by Ion Torrent DNA sequencing. Improved the time efficiency of iPSC-based feeder-free two-dimensional hematopoietic differentiation and evaluated inhibitors and cytokines, increasing production of hematopoietic cells. Investigated clonal potential of hematopoietic progenitors and used methylcellulose assays to assess lineage potential. Monitored differentiation of iPSC-derived hematopoietic cells and conducted in vivo assessments.